Prognostic significance of molecular-cytogenetic abnormalities in pediatric T-ALL is not explained by immunophenotypic differences

M van Grotel, J P P Meijerink, E R van Wering, A W Langerak, H B Beverloo, J G C A M Buijs-Gladdines, N B Burger, M Passier, E M van Lieshout, W A Kamps, A J P Veerman, Max van Noesel, R Pieters

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

Pediatric T-cell acute lymphoblastic leukemia (T-ALL) is characterized by chromosomal rearrangements possibly enforcing arrest at specific development stages. We studied the relationship between molecular-cytogenetic abnormalities and T-cell development stage to investigate whether arrest at specific stages can explain the prognostic significance of specific abnormalities. We extensively studied 72 pediatric T-ALL cases for genetic abnormalities and expression of transcription factors, NOTCH1 mutations and expression of specific CD markers. HOX11 cases were CD1 positive consistent with a cortical stage, but as 4/5 cases lacked cytoplasmatic-beta expression, developmental arrest may precede beta-selection. HOX11L2 was especially confined to immature and pre-AB developmental stages, but 3/17 HOX11L2 mature cases were restricted to the gammadelta-lineage. TAL1 rearrangements were restricted to the alphabeta-lineage with most cases being TCR-alphabeta positive. NOTCH1 mutations were present in all molecular-cytogenetic subgroups without restriction to a specific developmental stage. CALM-AF10 was associated with early relapse. TAL1 or HOX11L2 rearrangements were associated with trends to good and poor outcomes, respectively. Also cases with high vs low TAL1 expression levels demonstrated a trend toward good outcome. Most cases with lower TAL1 levels were HOX11L2 or CALM-AF10 positive. NOTCH1 mutations did not predict for outcome. Classification into T-cell developmental subgroups was not predictive for outcome.

Originele taal-2Engels
Pagina's (van-tot)124-31
Aantal pagina's8
TijdschriftLeukemia
Volume22
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - okt. 2007
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'Prognostic significance of molecular-cytogenetic abnormalities in pediatric T-ALL is not explained by immunophenotypic differences'. Samen vormen ze een unieke vingerafdruk.

Citeer dit