TY - JOUR
T1 - Rapid switching of TFIIH between RNA polymerase I and II transcription and DNA repair in vivo
AU - Hoogstraten, Deborah
AU - Nigg, Alex L.
AU - Heath, Helen
AU - Mullenders, Leon H.F.
AU - Van Driel, Roel
AU - Hoeijmakers, Jan H.J.
AU - Vermeulen, Wim
AU - Houtsmuller, Adriaan B.
N1 - Funding Information:
We thank Drs. A.A. van Zeeland, J. Essers, K. Mattern, and R. Kanaar for helpful suggestions and discussion and J.M. Egly for antibodies against TFIIH. This work was supported by the Dutch Scientific Organization (NWO-ALW and Spinoza award), the EC contract (DNA repair disorders), and the Dutch Cancer Society (KWF).
PY - 2002/11/1
Y1 - 2002/11/1
N2 - The transcription/repair factor TFIIH operates as a DNA helix opener in RNA polymerase II (RNAP2) transcription and nucleotide excision repair. To study TFIIH in vivo, we generated cell lines expressing functional GFP-tagged TFIIH. TFIIH was homogeneously distributed throughout the nucleus with nucleolar accumulations. We provide in vivo evidence for involvement of TFIIH in RNA polymerase I (RNAP1) transcription. Photobleaching revealed that TFIIH moves freely and gets engaged in RNAP1 and RNAP2 transcription for ∼25 and ∼6 s, respectively. TFIIH readily switches between transcription and repair sites (where it is immobilized for ∼4 min) without large-scale alterations in composition. Our findings support a model of diffusion and random collision of individual components that permits a quick and versatile response to changing conditions.
AB - The transcription/repair factor TFIIH operates as a DNA helix opener in RNA polymerase II (RNAP2) transcription and nucleotide excision repair. To study TFIIH in vivo, we generated cell lines expressing functional GFP-tagged TFIIH. TFIIH was homogeneously distributed throughout the nucleus with nucleolar accumulations. We provide in vivo evidence for involvement of TFIIH in RNA polymerase I (RNAP1) transcription. Photobleaching revealed that TFIIH moves freely and gets engaged in RNAP1 and RNAP2 transcription for ∼25 and ∼6 s, respectively. TFIIH readily switches between transcription and repair sites (where it is immobilized for ∼4 min) without large-scale alterations in composition. Our findings support a model of diffusion and random collision of individual components that permits a quick and versatile response to changing conditions.
UR - https://www.scopus.com/pages/publications/18744374129
U2 - 10.1016/S1097-2765(02)00709-8
DO - 10.1016/S1097-2765(02)00709-8
M3 - Article
C2 - 12453423
AN - SCOPUS:18744374129
SN - 1097-2765
VL - 10
SP - 1163
EP - 1174
JO - Molecular Cell
JF - Molecular Cell
IS - 5
ER -