@inbook{ea573741a80945d1b092827071103bb1,
title = "Reactive Oxygen Species (ROS) protection via cysteine oxidation in the epidermal cornified cell envelope",
abstract = "The outermost layer of our skin functions as a barrier to protect us from physical, chemical, and biological environmental insults. This protective function is mediated by the epidermal cornified cell envelope (CE) which serves both as a mechanical and permeability barrier. Recently we have discovered that the CE constitutes also a first-line antioxidant shield which relies greatly on cysteine residues in CE precursor proteins. Here we describe methods and protocols to study the cysteine-mediated antioxidant function of the CE at the level of the whole organ (the skin), individual cells (keratinocytes), or isolated proteins (SPRR family).",
keywords = "Cysteine/metabolism, Epidermal Cells, Humans, Keratinocytes/cytology, Mass Spectrometry, Molecular Imaging/methods, Oxidation-Reduction, Proteolysis, Reactive Oxygen Species/metabolism, Staining and Labeling, Trypsin/metabolism",
author = "Vermeij, {Wilbert P.} and Claude Backendorf",
year = "2014",
doi = "10.1007/7651_2013_51",
language = "English",
isbn = "9781493912230",
volume = "1195",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "157--169",
booktitle = "Methods in Molecular Biology",
}