TY - JOUR
T1 - Recognition of DNA damage by XPC coincides with disruption of the XPC-RAD23 complex
AU - Bergink, Steven
AU - Toussaint, Wendy
AU - Luijsterburg, Martijn S.
AU - Dinant, Christoffel
AU - Alekseev, Sergey
AU - Hoeijmakers, Jan H.J.
AU - Dantuma, Nico P.
AU - Houtsmuller, Adriaan B.
AU - Vermeulen, Wim
PY - 2012/3/19
Y1 - 2012/3/19
N2 - The recognition of helix-distorting deoxyribonucleic acid (DNA) lesions by the global genome nucleotide excision repair subpathway is performed by the XPC-RAD23-CEN2 complex. Although it has been established that Rad23 homologs are essential to protect XPC from proteasomal degradation, it is unclear whether RAD23 proteins have a direct role in the recognition of DNA damage. In this paper, we show that the association of XPC with ultraviolet-induced lesions was impaired in the absence of RAD23 proteins. Furthermore, we show that RAD23 proteins rapidly dissociated from XPC upon binding to damaged DNA. Our data suggest that RAD23 proteins facilitate lesion recognition by XPC but do not participate in the downstream DNA repair process.
AB - The recognition of helix-distorting deoxyribonucleic acid (DNA) lesions by the global genome nucleotide excision repair subpathway is performed by the XPC-RAD23-CEN2 complex. Although it has been established that Rad23 homologs are essential to protect XPC from proteasomal degradation, it is unclear whether RAD23 proteins have a direct role in the recognition of DNA damage. In this paper, we show that the association of XPC with ultraviolet-induced lesions was impaired in the absence of RAD23 proteins. Furthermore, we show that RAD23 proteins rapidly dissociated from XPC upon binding to damaged DNA. Our data suggest that RAD23 proteins facilitate lesion recognition by XPC but do not participate in the downstream DNA repair process.
UR - http://www.scopus.com/inward/record.url?scp=84860339685&partnerID=8YFLogxK
U2 - 10.1083/jcb.201107050
DO - 10.1083/jcb.201107050
M3 - Article
C2 - 22431748
AN - SCOPUS:84860339685
SN - 0021-9525
VL - 196
SP - 681
EP - 688
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
ER -