RhoA regulates translation of the Nogo-A decoy SPARC in white matter-invading glioblastomas

Peter Wirthschaft, Julia Bode, Himanshu Soni, Fabio Dietrich, Thomas Krüwel, Bernd Fischer, Christiane B. Knobbe-Thomsen, Giulia Rossetti, Andreas Hentschel, Norman Mack, Kai Schönig, Michael O. Breckwoldt, André Schmandke, Stefan Pusch, Jan Medenbach, Martin Bendszus, Martin E. Schwab, Andreas von Deimling, Marcel Kool, Christel Herold-MendeGuido Reifenberger, Robert Ahrends, Björn Tews

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

6 Citaten (Scopus)

Samenvatting

Glioblastomas strongly invade the brain by infiltrating into the white matter along myelinated nerve fiber tracts even though the myelin protein Nogo-A prevents cell migration by activating inhibitory RhoA signaling. The mechanisms behind this long-known phenomenon remained elusive so far, precluding a targeted therapeutic intervention. This study demonstrates that the prevalent activation of AKT in gliomas increases the ER protein-folding capacity and enables tumor cells to utilize a side effect of RhoA activation: the perturbation of the IRE1α-mediated decay of SPARC mRNA. Once translation is initiated, glioblastoma cells rapidly secrete SPARC to block Nogo-A from inhibiting migration via RhoA. By advanced ultramicroscopy for studying single-cell invasion in whole, undissected mouse brains, we show that gliomas require SPARC for invading into white matter structures. SPARC depletion reduces tumor dissemination that significantly prolongs survival and improves response to cytostatic therapy. Our finding of a novel RhoA-IRE1 axis provides a druggable target for interfering with SPARC production and underscores its therapeutic value.

Originele taal-2Engels
Pagina's (van-tot)275-293
Aantal pagina's19
TijdschriftActa Neuropathologica
Volume138
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - 1 aug. 2019
Extern gepubliceerdJa

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