TY - JOUR
T1 - RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
AU - Best, Myron G.
AU - Sol, Nik
AU - Kooi, Irsan
AU - Tannous, Jihane
AU - Westerman, Bart A.
AU - Rustenburg, François
AU - Schellen, Pepijn
AU - Verschueren, Heleen
AU - Post, Edward
AU - Koster, Jan
AU - Ylstra, Bauke
AU - Ameziane, Najim
AU - Dorsman, Josephine
AU - Smit, Egbert F.
AU - Verheul, Henk M.
AU - Noske, David P.
AU - Reijneveld, Jaap C.
AU - Nilsson, R. Jonas A.
AU - Tannous, Bakhos A.
AU - Wesseling, Pieter
AU - Wurdinger, Thomas
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/11/9
Y1 - 2015/11/9
N2 - Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based "liquid biopsies".
AB - Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based "liquid biopsies".
UR - http://www.scopus.com/inward/record.url?scp=84946568126&partnerID=8YFLogxK
U2 - 10.1016/j.ccell.2015.09.018
DO - 10.1016/j.ccell.2015.09.018
M3 - Article
C2 - 26525104
AN - SCOPUS:84946568126
SN - 1535-6108
VL - 28
SP - 666
EP - 676
JO - Cancer Cell
JF - Cancer Cell
IS - 5
ER -