Roadblock termination by reb1p restricts cryptic and readthrough transcription

Jessie Colin, Tito Candelli, Odil Porrua, Jocelyne Boulay, Chenchen Zhu, François Lacroute, Lars M. Steinmetz, Domenico Libri

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

46 Citaten (Scopus)

Samenvatting

Widely transcribed compact genomes must cope with the major challenge of frequent overlapping or concurrent transcription events. Efficient and timely transcription termination is crucial to control pervasive transcription and prevent transcriptional interference. In yeast, transcription termination of RNA polymerase II (RNAPII) occurs via two possible pathways that both require recognition of termination signals on nascent RNA by specific factors. We describe here an additional mechanism of transcription termination for RNAPII and demonstrate its biological significance. We show that the transcriptional activator Reb1p bound to DNA is a roadblock for RNAPII, which pauses and is ubiquitinated, thus triggering termination. Reb1p-dependent termination generates a class of cryptic transcripts that aredegraded in the nucleus by the exosome. We also observed transcriptional interference between neighboring genes in the absence of Reb1p. This work demonstrates the importance of roadblock termination for controlling pervasive transcription and preventing transcription through gene regulatory regions.

Originele taal-2Engels
Pagina's (van-tot)667-680
Aantal pagina's14
TijdschriftMolecular Cell
Volume56
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 4 dec. 2014
Extern gepubliceerdJa

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