SARS-CoV-2 productively infects human gut enterocytes

Mart M. Lamers; Joep Beumer; Jelte Van Der Vaart; Kèvin Knoops; Jens Puschhof; Tim I. Breugem; Raimond B.G. Ravelli; J. Paul Van Schayck; Anna Z. Mykytyn; Hans Q. Duimel; Elly Van Donselaar; Samra Riesebosch; Helma J.H. Kuijpers; Debby Schipper; Willine J.Van De Wetering; Miranda De Graaf; Marion Koopmans; Edwin Cuppen; Peter J. Peters; Bart L. Haagmans; Hans Clevers

doi:10.1126/science.abc1669

SARS-CoV-2 productively infects human gut enterocytes

Mart M. Lamers, Joep Beumer, Jelte Van Der Vaart, Kèvin Knoops, Jens Puschhof, Tim I. Breugem, Raimond B.G. Ravelli, J. Paul Van Schayck, Anna Z. Mykytyn, Hans Q. Duimel, Elly Van Donselaar, Samra Riesebosch, Helma J.H. Kuijpers, Debby Schipper, Willine J.Van De Wetering, Miranda De Graaf, Marion Koopmans, Edwin Cuppen, Peter J. Peters, Bart L. HaagmansHans Clevers

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.

Originele taal-2	Engels
Pagina's (van-tot)	50-54
Aantal pagina's	5
Tijdschrift	Science
Volume	369
Nummer van het tijdschrift	6499
DOI's	https://doi.org/10.1126/science.abc1669
Status	Gepubliceerd - 3 jul. 2020
Extern gepubliceerd	Ja

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10.1126/science.abc1669

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Lamers, M. M., Beumer, J., Vaart, J. V. D., Knoops, K., Puschhof, J., Breugem, T. I., Ravelli, R. B. G., Schayck, J. P. V., Mykytyn, A. Z., Duimel, H. Q., Donselaar, E. V., Riesebosch, S., Kuijpers, H. J. H., Schipper, D., Wetering, W. J. V. D., Graaf, M. D., Koopmans, M., Cuppen, E., Peters, P. J., ... Clevers, H. (2020). SARS-CoV-2 productively infects human gut enterocytes. Science, 369(6499), 50-54. https://doi.org/10.1126/science.abc1669

@article{b15a5aeabbae45258659fecfaa68a6f3,

title = "SARS-CoV-2 productively infects human gut enterocytes",

abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.",

author = "Lamers, {Mart M.} and Joep Beumer and Vaart, {Jelte Van Der} and K{\`e}vin Knoops and Jens Puschhof and Breugem, {Tim I.} and Ravelli, {Raimond B.G.} and Schayck, {J. Paul Van} and Mykytyn, {Anna Z.} and Duimel, {Hans Q.} and Donselaar, {Elly Van} and Samra Riesebosch and Kuijpers, {Helma J.H.} and Debby Schipper and Wetering, {Willine J.Van De} and Graaf, {Miranda De} and Marion Koopmans and Edwin Cuppen and Peters, {Peter J.} and Haagmans, {Bart L.} and Hans Clevers",

year = "2020",

month = jul,

day = "3",

doi = "10.1126/science.abc1669",

language = "English",

volume = "369",

pages = "50--54",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6499",

}

Lamers, MM, Beumer, J, Vaart, JVD, Knoops, K, Puschhof, J, Breugem, TI, Ravelli, RBG, Schayck, JPV, Mykytyn, AZ, Duimel, HQ, Donselaar, EV, Riesebosch, S, Kuijpers, HJH, Schipper, D, Wetering, WJVD, Graaf, MD, Koopmans, M, Cuppen, E, Peters, PJ, Haagmans, BL & Clevers, H 2020, 'SARS-CoV-2 productively infects human gut enterocytes', Science, vol. 369, nr. 6499, blz. 50-54. https://doi.org/10.1126/science.abc1669

TY - JOUR

T1 - SARS-CoV-2 productively infects human gut enterocytes

AU - Lamers, Mart M.

AU - Beumer, Joep

AU - Vaart, Jelte Van Der

AU - Knoops, Kèvin

AU - Puschhof, Jens

AU - Breugem, Tim I.

AU - Ravelli, Raimond B.G.

AU - Schayck, J. Paul Van

AU - Mykytyn, Anna Z.

AU - Duimel, Hans Q.

AU - Donselaar, Elly Van

AU - Riesebosch, Samra

AU - Kuijpers, Helma J.H.

AU - Schipper, Debby

AU - Wetering, Willine J.Van De

AU - Graaf, Miranda De

AU - Koopmans, Marion

AU - Cuppen, Edwin

AU - Peters, Peter J.

AU - Haagmans, Bart L.

AU - Clevers, Hans

PY - 2020/7/3

Y1 - 2020/7/3

N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.

AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.

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DO - 10.1126/science.abc1669

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C2 - 32358202

AN - SCOPUS:85087718106

SN - 0036-8075

VL - 369

SP - 50

EP - 54

JO - Science

JF - Science

IS - 6499

ER -

SARS-CoV-2 productively infects human gut enterocytes

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