SDHD immunohistochemistry: A new tool to validate SDHx mutations in pheochromocytoma/paraganglioma

Mélanie Menara, Lindsey Oudijk, Cécile Badoual, Jérôme Bertherat, Charlotte Lepoutre-Lussey, Laurence Amar, Xavier Iturrioz, Mathilde Sibony, Frank Zinzindohoué, Ronald De Krijger, Anne Paule Gimenez-Roqueplo, Judith Favier

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

48 Citaten (Scopus)

Samenvatting

Context: Pheochromocytomas (PCC) and paragangliomas (PGL) may be caused by a germline mutation in 12 different predisposing genes. We previously reported that immunohistochemistry is a useful approach to detect patients harboring SDHx mutations. SDHA immunostaining is negative in SDHA-mutated tumors only, while SDHB immunostaining is negative in samples mutated on all SDHx genes. In some cases of SDHD or SDHC-mutated tumors, a weak diffuse SDHB labeling has however been described. Objective: Here, we addressed whether the same procedure could be applicable to detect patients with germline SDHD mutations, by testing two new commercially available anti-SDHD antibodies. Design and Methods: We performed a retrospective study on 170 PGL/PCC in which we investigated SDHD and SDHB expression by immunohistochemistry. Results: SDHx-mutated PGL/PCC showed a completely negative SDHB staining (23/27) or a weak cytoplasmic background (4/27). Unexpectedly, weobserved that SDHD immunohistochemistry was positive in SDHx-deficient tumors and negative in the other samples. Twenty-six of 27 SDHx tumors (including the four weakly stained for SDHB) were positive for SDHD. Among non-SDHx tumors, 138/143 were positive for SDHB and negative for SDHD. Five cases showed a negative immuno-staining for SDHB, but were negative for SDHD. Conclusion: Our results demonstrate that a positive SDHD immunostaining predicts the presence of an SDHx gene mutation. Because SDHB negative immunostaining is sometimes difficult to interpret in the case of background, the addition of SDHD positive immunohistochemistry will be a very useful tool to predict or validate SDHx gene variants in PGL/PCC.

Originele taal-2Engels
Pagina's (van-tot)E287-E291
TijdschriftJournal of Clinical Endocrinology and Metabolism
Volume100
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - 1 feb. 2015
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'SDHD immunohistochemistry: A new tool to validate SDHx mutations in pheochromocytoma/paraganglioma'. Samen vormen ze een unieke vingerafdruk.

Citeer dit