TY - JOUR
T1 - Separation of neural and surface ectoderm after closure of the rostral neuropore
AU - Hoving, E W
AU - Vermeij-Keers, C
AU - Mommaas-Kienhuis, A M
AU - Hartwig, N G
PY - 1990
Y1 - 1990
N2 - Separation of neural and surface ectoderm after closure of the rostral neuropore in the head region has been described by investigating the integrity of the basement membranes of these epithelia in 11- to 27-somite rat embryos. The basement membranes were visualized with polyclonal antibodies against laminin. Furthermore, cell degeneration has been investigated in relation to neural crest activity, and discontinuities of the basement membrane in 9- to 30-somite mouse embryos. The separation of the basement membranes of neural and surface ectoderm in the midline is a final phase during the fusion of the neural folds, which takes place from the closure of the rostral neuropore, at the 19-somite stage, until the 27-somite stage (rat embryos), and which occurs focally with variation in the midsagittal and the transverse planes. In the prosencephalon, neural crest activity is absent during the separation phase of both epithelia, but cell degeneration may contribute to the separation of the initially connected basement membranes. A disturbance in the separation of the neural and surface ectoderm may be the pathogenetic basis of midline skull defects, and of the fronto-ethmoidal encephalocele in particular.
AB - Separation of neural and surface ectoderm after closure of the rostral neuropore in the head region has been described by investigating the integrity of the basement membranes of these epithelia in 11- to 27-somite rat embryos. The basement membranes were visualized with polyclonal antibodies against laminin. Furthermore, cell degeneration has been investigated in relation to neural crest activity, and discontinuities of the basement membrane in 9- to 30-somite mouse embryos. The separation of the basement membranes of neural and surface ectoderm in the midline is a final phase during the fusion of the neural folds, which takes place from the closure of the rostral neuropore, at the 19-somite stage, until the 27-somite stage (rat embryos), and which occurs focally with variation in the midsagittal and the transverse planes. In the prosencephalon, neural crest activity is absent during the separation phase of both epithelia, but cell degeneration may contribute to the separation of the initially connected basement membranes. A disturbance in the separation of the neural and surface ectoderm may be the pathogenetic basis of midline skull defects, and of the fronto-ethmoidal encephalocele in particular.
KW - Animals
KW - Basement Membrane/metabolism
KW - Cell Movement/physiology
KW - Cell Survival/physiology
KW - Ectoderm/cytology
KW - Embryo, Mammalian/cytology
KW - Epidermal Cells
KW - Epidermis/embryology
KW - Epithelial Cells
KW - Epithelium/physiology
KW - Histocytochemistry
KW - Laminin/metabolism
KW - Mesencephalon/cytology
KW - Mice
KW - Microscopy, Electron
KW - Morphogenesis/physiology
KW - Nervous System/cytology
KW - Neural Crest/cytology
KW - Oxazines
KW - Rats
KW - Rats, Inbred Strains
KW - Rhombencephalon/cytology
U2 - 10.1007/BF00178910
DO - 10.1007/BF00178910
M3 - Article
C2 - 2291490
SN - 0340-2061
VL - 182
SP - 455
EP - 463
JO - Anatomy and embryology
JF - Anatomy and embryology
IS - 5
ER -