Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis

Willy M. Baarends, Evelyne Wassenaar, Roald Van Der Laan, Jos Hoogerbrugge, Esther Sleddens-Linkels, Jan H.J. Hoeijmakers, Peter De Boer, J. Anton Grootegoed

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

255 Citaten (Scopus)


During meiotic prophase in male mammals, the X and Y chromosomes are incorporated in the XY body. This heterochromatic body is transcriptionally silenced and marked by increased ubiquitination of histone H2A. This led us to investigate the relationship between histone H2A ubiquitination and chromatin silencing in more detail. First, we found that ubiquitinated H2A also marks the silenced X chromosome of the Barr body in female somatic cells. Next, we studied a possible relationship between H2A ubiquitination, chromatin silencing, and unpaired chromatin in meiotic prophase. The mouse models used carry an unpaired autosomal region in male meiosis or unpaired X and Y chromosomes in female meiosis. We show that ubiquitinated histone H2A is associated with transcriptional silencing of large chromatin regions. This silencing in mammalian meiotic prophase cells concerns unpaired chromatin regions and resembles a phenomenon described for the fungus Neurospora crassa and named meiotic silencing by unpaired DNA.

Originele taal-2Engels
Pagina's (van-tot)1041-1053
Aantal pagina's13
TijdschriftMolecular and Cellular Biology
Nummer van het tijdschrift3
StatusGepubliceerd - feb. 2005
Extern gepubliceerdJa


Duik in de onderzoeksthema's van 'Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis'. Samen vormen ze een unieke vingerafdruk.

Citeer dit