TY - JOUR
T1 - Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis
AU - Baarends, Willy M.
AU - Wassenaar, Evelyne
AU - Van Der Laan, Roald
AU - Hoogerbrugge, Jos
AU - Sleddens-Linkels, Esther
AU - Hoeijmakers, Jan H.J.
AU - De Boer, Peter
AU - Grootegoed, J. Anton
PY - 2005/2
Y1 - 2005/2
N2 - During meiotic prophase in male mammals, the X and Y chromosomes are incorporated in the XY body. This heterochromatic body is transcriptionally silenced and marked by increased ubiquitination of histone H2A. This led us to investigate the relationship between histone H2A ubiquitination and chromatin silencing in more detail. First, we found that ubiquitinated H2A also marks the silenced X chromosome of the Barr body in female somatic cells. Next, we studied a possible relationship between H2A ubiquitination, chromatin silencing, and unpaired chromatin in meiotic prophase. The mouse models used carry an unpaired autosomal region in male meiosis or unpaired X and Y chromosomes in female meiosis. We show that ubiquitinated histone H2A is associated with transcriptional silencing of large chromatin regions. This silencing in mammalian meiotic prophase cells concerns unpaired chromatin regions and resembles a phenomenon described for the fungus Neurospora crassa and named meiotic silencing by unpaired DNA.
AB - During meiotic prophase in male mammals, the X and Y chromosomes are incorporated in the XY body. This heterochromatic body is transcriptionally silenced and marked by increased ubiquitination of histone H2A. This led us to investigate the relationship between histone H2A ubiquitination and chromatin silencing in more detail. First, we found that ubiquitinated H2A also marks the silenced X chromosome of the Barr body in female somatic cells. Next, we studied a possible relationship between H2A ubiquitination, chromatin silencing, and unpaired chromatin in meiotic prophase. The mouse models used carry an unpaired autosomal region in male meiosis or unpaired X and Y chromosomes in female meiosis. We show that ubiquitinated histone H2A is associated with transcriptional silencing of large chromatin regions. This silencing in mammalian meiotic prophase cells concerns unpaired chromatin regions and resembles a phenomenon described for the fungus Neurospora crassa and named meiotic silencing by unpaired DNA.
UR - http://www.scopus.com/inward/record.url?scp=12844280588&partnerID=8YFLogxK
U2 - 10.1128/MCB.25.3.1041-1053.2005
DO - 10.1128/MCB.25.3.1041-1053.2005
M3 - Article
C2 - 15657431
AN - SCOPUS:12844280588
SN - 0270-7306
VL - 25
SP - 1041
EP - 1053
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 3
ER -