TY - JOUR
T1 - Simultaneous determination of the azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in human plasma by reversed phase ultra-performance liquid chromatography with ultraviolet detection
AU - Verweij-van Wissen, C. P.W.G.M.
AU - Burger, D. M.
AU - Verweij, P. E.
AU - Aarnoutse, R. E.
AU - Brüggemann, R. J.M.
PY - 2012/3/1
Y1 - 2012/3/1
N2 - A reversed phase ultra-performance liquid chromatography method was developed for the simultaneous quantitative determination of the systemically administered azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in plasma. The method involved a simple liquid-liquid extraction followed by ultra-performance liquid chromatography with an Acquity UPLC BEH Phenyl column and ultraviolet detection set at a wavelength of 260. nm. The assay was validated over the concentration range of 0.050-10,000. mg/l for all four azoles and one metabolite. The accuracies for the assay were 97-104%, inter- and intra-day coefficients of variation were <7.5% and extraction recoveries were 98% for voriconazole, posaconazole, isavuconazole and the internal standard (UK115794), and 79% and 88% for itraconazole and hydroxy-itraconazole respectively. This method proved to be simple, accurate, precise and fast and is currently in use in our laboratory for the quantitative analysis of these azoles for Therapeutic Drug Monitoring and pharmacokinetic research.
AB - A reversed phase ultra-performance liquid chromatography method was developed for the simultaneous quantitative determination of the systemically administered azoles voriconazole, posaconazole, isavuconazole, itraconazole and its metabolite hydroxy-itraconazole in plasma. The method involved a simple liquid-liquid extraction followed by ultra-performance liquid chromatography with an Acquity UPLC BEH Phenyl column and ultraviolet detection set at a wavelength of 260. nm. The assay was validated over the concentration range of 0.050-10,000. mg/l for all four azoles and one metabolite. The accuracies for the assay were 97-104%, inter- and intra-day coefficients of variation were <7.5% and extraction recoveries were 98% for voriconazole, posaconazole, isavuconazole and the internal standard (UK115794), and 79% and 88% for itraconazole and hydroxy-itraconazole respectively. This method proved to be simple, accurate, precise and fast and is currently in use in our laboratory for the quantitative analysis of these azoles for Therapeutic Drug Monitoring and pharmacokinetic research.
KW - Analytical
KW - Antifungal
KW - Pharmacokinetics
KW - Therapeutic drug monitoring
UR - http://www.scopus.com/inward/record.url?scp=84857141568&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2012.01.015
DO - 10.1016/j.jchromb.2012.01.015
M3 - Article
C2 - 22336695
AN - SCOPUS:84857141568
SN - 1570-0232
VL - 887-888
SP - 79
EP - 84
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -