TY - JOUR
T1 - SOX9 Is Required for the Differentiation of Paneth Cells in the Intestinal Epithelium
AU - Mori-Akiyama, Yuko
AU - van den Born, Maaike
AU - van Es, Johan H.
AU - Hamilton, Stanley R.
AU - Adams, Henry P.
AU - Zhang, Jiexin
AU - Clevers, Hans
AU - de Crombrugghe, Benoit
PY - 2007/8
Y1 - 2007/8
N2 - Background & Aims: The transcription factor SOX9 has been shown previously to have an essential role in the differentiation of a small number of discrete cell lineages. In the intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt signaling. Methods: To examine the function of SOX9 in the intestine, we inactivated the Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9 gene and a Villin-Cre transgene. Results: In the absence of SOX9, Paneth cells were not formed, but the differentiation of other intestinal epithelial cell types was unaffected. The lack of SOX9 also lead to crypt enlargement, to a marked increase in cell proliferation throughout the crypts, and to replacement of the Paneth cells by proliferating epithelial cells. Conclusions: We conclude that SOX9 is required for the differentiation of Paneth cells. Our results elucidate an essential step in the differentiation of gut epithelium.
AB - Background & Aims: The transcription factor SOX9 has been shown previously to have an essential role in the differentiation of a small number of discrete cell lineages. In the intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt signaling. Methods: To examine the function of SOX9 in the intestine, we inactivated the Sox9 gene in intestinal epithelial cells by generating mice that harbored a conditional Sox9 gene and a Villin-Cre transgene. Results: In the absence of SOX9, Paneth cells were not formed, but the differentiation of other intestinal epithelial cell types was unaffected. The lack of SOX9 also lead to crypt enlargement, to a marked increase in cell proliferation throughout the crypts, and to replacement of the Paneth cells by proliferating epithelial cells. Conclusions: We conclude that SOX9 is required for the differentiation of Paneth cells. Our results elucidate an essential step in the differentiation of gut epithelium.
UR - http://www.scopus.com/inward/record.url?scp=34547549553&partnerID=8YFLogxK
U2 - 10.1053/j.gastro.2007.05.020
DO - 10.1053/j.gastro.2007.05.020
M3 - Article
C2 - 17681175
AN - SCOPUS:34547549553
SN - 0016-5085
VL - 133
SP - 539
EP - 546
JO - Gastroenterology
JF - Gastroenterology
IS - 2
ER -