TY - JOUR
T1 - Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6
AU - Koken, Marcel H.M.
AU - Reynolds, Paul
AU - Jaspers-Dekker, Iris
AU - Prakash, Louise
AU - Prakash, Satya
AU - Bootsma, Dirk
AU - Hoeijmakers, Jan H.J.
PY - 1991/10/15
Y1 - 1991/10/15
N2 - The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and ≈70% and ≈85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possesses the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6Δ mutants.
AB - The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and ≈70% and ≈85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possesses the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6Δ mutants.
KW - DNA damage
KW - E2 enzyme
KW - Sporulation
KW - Ubiquitin conjugation
KW - UV mutagenesis
UR - http://www.scopus.com/inward/record.url?scp=0025998528&partnerID=8YFLogxK
U2 - 10.1073/pnas.88.20.8865
DO - 10.1073/pnas.88.20.8865
M3 - Article
C2 - 1717990
AN - SCOPUS:0025998528
SN - 0027-8424
VL - 88
SP - 8865
EP - 8869
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -