Structure-system correlation identifies a gene regulatory Mediator submodule

Laurent Larivière, Martin Seizl, Sake Van Wageningen, Susanne Röther, Loes Van De Pasch, Heidi Feldmann, Katja Sträßer, Steve Hahn, Frank C.P. Holstege, Patrick Cramer

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

32 Citaten (Scopus)

Samenvatting

A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved α-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, genespecific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.

Originele taal-2Engels
Pagina's (van-tot)872-877
Aantal pagina's6
TijdschriftGenes and Development
Volume22
Nummer van het tijdschrift7
DOI's
StatusGepubliceerd - 1 apr. 2008
Extern gepubliceerdJa

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