Samenvatting
We have sublocalized the human proto-oncogene c-myb by applying two different techniques: In situ hybridization of metaphase spreads and chromosome spot hybridization of flow-sorted chromosomes. For this we used a teratocarcinoma cell line carrying specific chromosome translocations involving the two chromosomes 6 and one chromosome 11. The distribution of the c-myb gene copies on the different translocation chromosomes revealed that c-myb is located in the region 6q21→q23. Because of the close proximity of the c-myb locus to the chromosomal breakpoints in the teratocarcinoma, we investigated whether c-myb was implicated in the development of this tumor. No rearrangement, deletion, or amplification of the gene was detected in the teratocarcinoma cells. Furthermore, the level of c-myb expression was comparable to that of other cell lines of nonhematopoietic origin. These results suggest that c-myb was not affected by the translocation and played no significant role in the development of this teratocarcinoma.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 129-135 |
Aantal pagina's | 7 |
Tijdschrift | Cytogenetic and Genome Research |
Volume | 41 |
Nummer van het tijdschrift | 3 |
DOI's | |
Status | Gepubliceerd - 1986 |
Extern gepubliceerd | Ja |