Sublocalization of c-myb to 6q21→q23 by in situ hybridization and c-myb expression in a human teratocarcinoma with 6q rearrangements

J. W.G. Janssen, P. Vernole, P. A.J. De Boer, J. W. Oosterhuis, John G. Collard

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27 Citaten (Scopus)

Samenvatting

We have sublocalized the human proto-oncogene c-myb by applying two different techniques: In situ hybridization of metaphase spreads and chromosome spot hybridization of flow-sorted chromosomes. For this we used a teratocarcinoma cell line carrying specific chromosome translocations involving the two chromosomes 6 and one chromosome 11. The distribution of the c-myb gene copies on the different translocation chromosomes revealed that c-myb is located in the region 6q21→q23. Because of the close proximity of the c-myb locus to the chromosomal breakpoints in the teratocarcinoma, we investigated whether c-myb was implicated in the development of this tumor. No rearrangement, deletion, or amplification of the gene was detected in the teratocarcinoma cells. Furthermore, the level of c-myb expression was comparable to that of other cell lines of nonhematopoietic origin. These results suggest that c-myb was not affected by the translocation and played no significant role in the development of this teratocarcinoma.

Originele taal-2Engels
Pagina's (van-tot)129-135
Aantal pagina's7
TijdschriftCytogenetic and Genome Research
Volume41
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 1986
Extern gepubliceerdJa

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