The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma

Martin Hagenaars, Ron Koelemij, N. Geeske Ensink, Jaap D.H. Van Eendenburg, Ronald L.P. Van Vlierberghe, Alexander M.M. Eggermont, Cornelis J.H. Van de Velde, Gert Jan Fleuren, Peter J.K. Kuppen

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

26 Citaten (Scopus)

Samenvatting

In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.

Originele taal-2Engels
Pagina's (van-tot)281-289
Aantal pagina's9
TijdschriftClinical and Experimental Metastasis
Volume18
Nummer van het tijdschrift4
DOI's
StatusGepubliceerd - 2000
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma'. Samen vormen ze een unieke vingerafdruk.

Citeer dit