TY - JOUR
T1 - The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma
AU - Hagenaars, Martin
AU - Koelemij, Ron
AU - Ensink, N. Geeske
AU - Van Eendenburg, Jaap D.H.
AU - Van Vlierberghe, Ronald L.P.
AU - Eggermont, Alexander M.M.
AU - Van de Velde, Cornelis J.H.
AU - Fleuren, Gert Jan
AU - Kuppen, Peter J.K.
N1 - Funding Information:
This work was supported by the Dutch Cancer Society, grant number RUL 94-784. We also thank Mrs H.A.M. Holtslag and Mrs M. van Brakel for excellent technical assistance.
PY - 2000
Y1 - 2000
N2 - In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.
AB - In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.
KW - Colon tumour
KW - Monoclonal antibody
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0034961058&partnerID=8YFLogxK
U2 - 10.1023/A:1011062002851
DO - 10.1023/A:1011062002851
M3 - Article
C2 - 11448057
AN - SCOPUS:0034961058
SN - 0262-0898
VL - 18
SP - 281
EP - 289
JO - Clinical and Experimental Metastasis
JF - Clinical and Experimental Metastasis
IS - 4
ER -