The ERCC2/DNA repair protein is associated with the class II BTF2/TFIIH transcription factor

L. Schaeffer, V. Moncollin, R. Roy, A. Staub, M. Mezzina, A. Sarasin, G. Weeda, J. H.J. Hoeijmakers, J. M. Egly

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345 Citaten (Scopus)

Samenvatting

ERCC2 is involved in the DNA repair syndrome xeroderma pigmentosum (XP) group D and was found to copurify with the RNA polymerase II (B) transcription factor BTF2/TEIIH that possesses a bidirectional helicase activity. Antibodies directed towards the 89 kDa (ERCC3) or the p62 subunit of BTF2 are able to either immunoprecipitate ERCC2 or shift the polypeptide in a glycerol gradient. Conversely, an antibody directed towards ERCC2 also retains or shifts BTF2. ERCC2 could be resolved from the other characterized components of BTF2 upon salt treatment, while its readdition enhanced BTF2 transcription activity. ERCC2, ERCC3 and p44 are three repair proteins found in association with BTF2. Two of them, ERCC2 and ERCC3, are responsible for atypical forms of XP disorders which confer a high predisposition to skin cancer. This includes clinical features that lack an adequate rationalization on the basis of nucleotide excision repair (NER) deficiency but which may now be explained better in terms of a partial transcription deficiency.

Originele taal-2Engels
Pagina's (van-tot)2388-2392
Aantal pagina's5
TijdschriftEMBO Journal
Volume13
Nummer van het tijdschrift10
DOI's
StatusGepubliceerd - 1994
Extern gepubliceerdJa

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