The Intestinal Wnt/TCF Signature

Laurens G. Van der Flier, Jacob Sabates-Bellver, Irma Oving, Andrea Haegebarth, Mariagrazia De Palo, Marcello Anti, Marielle E. Van Gijn, Saskia Suijkerbuijk, Marc Van de Wetering, Giancarlo Marra, Hans Clevers

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411 Citaten (Scopus)

Samenvatting

Background & Aims: In colorectal cancer, activating mutations in the Wnt pathway transform epithelial cells through the inappropriate expression of a TCF4 target gene program, which is physiologically expressed in intestinal crypts. Methods: We have now performed an exhaustive array-based analysis of this target gene program in colorectal cancer cell lines carrying an inducible block of the Wnt cascade. Independently, differential gene-expression profiles of human adenomas and adenocarcinomas vs normal colonic epithelium were obtained. Results Expression analyses of approximately 80 genes common between these data sets were performed in a murine adenoma model. The combined data sets describe a core target gene program, the intestinal Wnt/TCF signature gene set, which is responsible for the transformation of human intestinal epithelial cells. Conclusions The genes were invariably expressed in adenomas, yet could be subdivided into 3 modules, based on expression in distinct crypt compartments. A module of 17 genes was specifically expressed at the position of the crypt stem cell.

Originele taal-2Engels
Pagina's (van-tot)628-632
Aantal pagina's5
TijdschriftGastroenterology
Volume132
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - feb. 2007
Extern gepubliceerdJa

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