TY - JOUR
T1 - The kinetoplast DNA of Trypanosoma equiperdum
AU - Frasch, A. C.C.
AU - Hajduk, S. L.
AU - Hoeijmakers, J. H.J.
AU - Borst, P.
AU - Brunel, F.
AU - Davidson, J.
N1 - Funding Information:
We thank Mrs. F. Fase-Fowler and Miss J. Van den Burg for their help in some of these experiments and Mr. J. Hoffschlag and Mr. F.P.J.M. Leijse for running the ultracentrifuge. We are indebted to the Staff of the Laboratory of Electron Microscopy (University of Amsterdam) for their generous provision of facilities. A.C.C.F. gratefully acknowledges a research training grant from the World Health Organization (No. MS/181/4/F.78). This work was supported in part by a grant to P.B. from the Foundation for Fundamental Biological Research (BION) which is subsidized by the Netherlands Organization for the Advancement of Pure Research (ZWO) and a grant from the North Atlantic Treaty Organization (No. 559).
PY - 1980/5/30
Y1 - 1980/5/30
N2 - We have analyzed the kinetoplast DNA from Trypanosoma equiperdum (American Type Culture Collection 30019) and two dyskinetoplastic strains derived from it. The DNA networks from the kinetoplastic strain are made up of catenated mini-circles and maxi-circles, like the networks from the closely-related Trypanosoma brucei. The mini-circles of T. equiperdum lack the pronounced sequence heterogeneity of T. brucei mini-circles, as shown by the fragment distribution of restriction digests and by the predominance of well-matched duplexes in electron micrographs of renatured DNA. The electrophoretic analysis of kinetoplast DNA digested with various restriction endonucleases shows the maxi-circle of T. equiperdum to consist of circular DNA molecules of 8.4 · 106 daltons, without size or sequence heterogeneity or repetitious segments. A comparison of the sequence of this maxi-circle with that of T. brucei (13.4 · 106 daltons) by restriction endonuclease fragmentation and hybridization shows extensive sequence homology. The size difference between both maxi-circles is due to the deletion of one continuous segment of 5 · 106 daltons. In the two dyskinetoplastic strains, we cannot detect DNA sequences that hybridize with kinetoplast DNA from T. brucei or from the kinetoplastic strain of T. equiperdum. In one of these strains, a 'low-density' DNA fraction contained a simple sequence DNA, cleaved by restriction endonuclease HindIII into fragments of 180 base-pairs and multimers of this. The relation of this DNA to kinetoplast DNA, if any, is unknown.
AB - We have analyzed the kinetoplast DNA from Trypanosoma equiperdum (American Type Culture Collection 30019) and two dyskinetoplastic strains derived from it. The DNA networks from the kinetoplastic strain are made up of catenated mini-circles and maxi-circles, like the networks from the closely-related Trypanosoma brucei. The mini-circles of T. equiperdum lack the pronounced sequence heterogeneity of T. brucei mini-circles, as shown by the fragment distribution of restriction digests and by the predominance of well-matched duplexes in electron micrographs of renatured DNA. The electrophoretic analysis of kinetoplast DNA digested with various restriction endonucleases shows the maxi-circle of T. equiperdum to consist of circular DNA molecules of 8.4 · 106 daltons, without size or sequence heterogeneity or repetitious segments. A comparison of the sequence of this maxi-circle with that of T. brucei (13.4 · 106 daltons) by restriction endonuclease fragmentation and hybridization shows extensive sequence homology. The size difference between both maxi-circles is due to the deletion of one continuous segment of 5 · 106 daltons. In the two dyskinetoplastic strains, we cannot detect DNA sequences that hybridize with kinetoplast DNA from T. brucei or from the kinetoplastic strain of T. equiperdum. In one of these strains, a 'low-density' DNA fraction contained a simple sequence DNA, cleaved by restriction endonuclease HindIII into fragments of 180 base-pairs and multimers of this. The relation of this DNA to kinetoplast DNA, if any, is unknown.
KW - (Trypanosoma equiperdum)
KW - Kinetoplast DNA
KW - Maxi-circle
KW - Mini-circle
UR - http://www.scopus.com/inward/record.url?scp=0019312048&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(80)90150-1
DO - 10.1016/0005-2787(80)90150-1
M3 - Article
C2 - 6249368
AN - SCOPUS:0019312048
SN - 0005-2787
VL - 607
SP - 397
EP - 410
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 3
ER -