TY - JOUR
T1 - The major transcripts of the kinetoplast DNA of Trypanosoma brucei are very small ribosomal RNAs
AU - Eperon, I. C.
AU - Janssen, J. W.G.
AU - Hoeijmakers, J. H.J.
AU - Borst, P.
N1 - Funding Information:
Acknowledgements We would like to thank F. Sanger for advice and encouragement, A.T. Bankier for communicating the optimum conditions for digestion of DNA with EcoRI* activity and H.F. Noller for communicating a possible secondary structure for the human and bovine 12S rRNAs. This work was supported in part by a grant to P.B. from the North Atlantic Treaty Organisation (NATO-559).
PY - 1983/1/11
Y1 - 1983/1/11
N2 - The nucleotide sequence has been determined of a 2.2 kb segment of kinetoplast DNA, which encodes the major mitochondrial transcripts (12S and 9S) of Trypanosoma brucei. The sequence shows that the 12S RNA is a large subunit rRNA, although sufficiently unusual for resistance to chloramphenicol to be predicted. The 9S RNA has little homology with other rRNAs, but a possible secondary structure is not unlike that of the 2.5-fold larger E.coli 16S rRNA. We conclude that the 12S RNA (about 1230 nucleotides) and the 9S RNA (about 640 nucleotides) are the smallest homologues of the E.coli 23S and 16S rRNAs yet observed.
AB - The nucleotide sequence has been determined of a 2.2 kb segment of kinetoplast DNA, which encodes the major mitochondrial transcripts (12S and 9S) of Trypanosoma brucei. The sequence shows that the 12S RNA is a large subunit rRNA, although sufficiently unusual for resistance to chloramphenicol to be predicted. The 9S RNA has little homology with other rRNAs, but a possible secondary structure is not unlike that of the 2.5-fold larger E.coli 16S rRNA. We conclude that the 12S RNA (about 1230 nucleotides) and the 9S RNA (about 640 nucleotides) are the smallest homologues of the E.coli 23S and 16S rRNAs yet observed.
UR - http://www.scopus.com/inward/record.url?scp=0021100533&partnerID=8YFLogxK
U2 - 10.1093/nar/11.1.105
DO - 10.1093/nar/11.1.105
M3 - Article
C2 - 6306559
AN - SCOPUS:0021100533
SN - 0305-1048
VL - 11
SP - 105
EP - 125
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 1
ER -