The MicroRNA-371 Family as Plasma Biomarkers for Monitoring Undifferentiated and Potentially Malignant Human Pluripotent Stem Cells in Teratoma Assays

Daniela C F Salvatori, Lambert C J Dorssers, Ad J M Gillis, Gemma Perretta, Ton van Agthoven, Maria Gomes Fernandes, Hans Stoop, Jan-Bas Prins, J Wolter Oosterhuis, Christine Mummery, Leendert H J Looijenga

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Samenvatting

Predicting developmental potency and risk of posttransplantation tumor formation by human pluripotent stem cells (hPSCs) and their derivatives largely rely on classical histological analysis of teratomas. Here, we investigated whether an assay based on microRNAs (miRNA) in blood plasma is able to detect potentially malignant elements. Several hPSCs and human malignant germ cell tumor (hGCT) lines were investigated in vitro and in vivo after mouse xenografting. The multiple conventional hPSC lines generated mature teratomas, while xenografts from induced hPSCs (hiPSCs) with reactivated reprogramming transgenes and hGCT lines contained undifferentiated and potentially malignant components. The presence of these elements was reflected in the mRNA and miRNA profiles of the xenografts with OCT3/4 mRNA and the miR-371 and miR-302 families readily detectable. miR-371 family members were also identified in mouse plasma faithfully reporting undifferentiated elements in the xenografts. This study demonstrated that undifferentiated and potentially malignant cells could be detected in vivo.

Originele taal-2Engels
Pagina's (van-tot)1493-1505
Aantal pagina's13
TijdschriftStem Cell Reports
Volume11
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 11 dec. 2018
Extern gepubliceerdJa

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