TY - JOUR
T1 - The Sox-13 gene
T2 - Structure, promoter characterization, and chromosomal localization
AU - Roose, Jeroen
AU - Korver, Wouter
AU - De Boer, Rosalie
AU - Kuipers, Jeroen
AU - Hurenkamp, Jolanda
AU - Clevers, Hans
N1 - Funding Information:
The authors thank Dr. J. van Es and Dr. R. Medema, for critically reading the manuscript, and the members of the Clevers laboratory for helpful discussions. This project was supported by a PIONIER grant from NWO-GMW.
PY - 1999/4/15
Y1 - 1999/4/15
N2 - We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.
AB - We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.
UR - http://www.scopus.com/inward/record.url?scp=0345620819&partnerID=8YFLogxK
U2 - 10.1006/geno.1999.5779
DO - 10.1006/geno.1999.5779
M3 - Article
C2 - 10198172
AN - SCOPUS:0345620819
SN - 0888-7543
VL - 57
SP - 301
EP - 305
JO - Genomics
JF - Genomics
IS - 2
ER -