The Sox-13 gene: Structure, promoter characterization, and chromosomal localization

Jeroen Roose; Wouter Korver; Rosalie De Boer; Jeroen Kuipers; Jolanda Hurenkamp; Hans Clevers

doi:10.1006/geno.1999.5779

The Sox-13 gene: Structure, promoter characterization, and chromosomal localization

Jeroen Roose, Wouter Korver, Rosalie De Boer, Jeroen Kuipers, Jolanda Hurenkamp, Hans Clevers

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

16 Citaten (Scopus)

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We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.

Originele taal-2	Engels
Pagina's (van-tot)	301-305
Aantal pagina's	5
Tijdschrift	Genomics
Volume	57
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1006/geno.1999.5779
Status	Gepubliceerd - 15 apr. 1999
Extern gepubliceerd	Ja

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10.1006/geno.1999.5779

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title = "The Sox-13 gene: Structure, promoter characterization, and chromosomal localization",

abstract = "We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.",

author = "Jeroen Roose and Wouter Korver and {De Boer}, Rosalie and Jeroen Kuipers and Jolanda Hurenkamp and Hans Clevers",

note = "Funding Information: The authors thank Dr. J. van Es and Dr. R. Medema, for critically reading the manuscript, and the members of the Clevers laboratory for helpful discussions. This project was supported by a PIONIER grant from NWO-GMW.",

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AU - Kuipers, Jeroen

AU - Hurenkamp, Jolanda

AU - Clevers, Hans

N1 - Funding Information: The authors thank Dr. J. van Es and Dr. R. Medema, for critically reading the manuscript, and the members of the Clevers laboratory for helpful discussions. This project was supported by a PIONIER grant from NWO-GMW.

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N2 - We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.

AB - We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.

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The Sox-13 gene: Structure, promoter characterization, and chromosomal localization

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