The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters

Adalberto Costessi, Nawel Mahrour, Esther Tijchon, Rieka Stunnenberg, Marieke A. Stoel, Pascal W. Jansen, Dotan Sela, Skylar Martin-Brown, Michael P. Washburn, Laurence Florens, Joan W. Conaway, Ronald C. Conaway, Hendrik G. Stunnenberg

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54 Citaten (Scopus)

Samenvatting

The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumourigenesis remain largely elusive. We applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PRAME can be recruited to DNA in vitro, and genome-wide chromatin immunoprecipitation experiments revealed that PRAME is specifically enriched at transcriptionally active promoters that are also bound by NFY and at enhancers. Our results are consistent with a role for the PRAME ubiquitin ligase complex in NFY-mediated transcriptional regulation.

Originele taal-2Engels
Pagina's (van-tot)3786-3798
Aantal pagina's13
TijdschriftEMBO Journal
Volume30
Nummer van het tijdschrift18
DOI's
StatusGepubliceerd - sep. 2011
Extern gepubliceerdJa

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