The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters

Adalberto Costessi; Nawel Mahrour; Esther Tijchon; Rieka Stunnenberg; Marieke A. Stoel; Pascal W. Jansen; Dotan Sela; Skylar Martin-Brown; Michael P. Washburn; Laurence Florens; Joan W. Conaway; Ronald C. Conaway; Hendrik G. Stunnenberg

doi:10.1038/emboj.2011.262

The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters

Adalberto Costessi, Nawel Mahrour, Esther Tijchon, Rieka Stunnenberg, Marieke A. Stoel, Pascal W. Jansen, Dotan Sela, Skylar Martin-Brown, Michael P. Washburn, Laurence Florens, Joan W. Conaway, Ronald C. Conaway, Hendrik G. Stunnenberg

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

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The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumourigenesis remain largely elusive. We applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PRAME can be recruited to DNA in vitro, and genome-wide chromatin immunoprecipitation experiments revealed that PRAME is specifically enriched at transcriptionally active promoters that are also bound by NFY and at enhancers. Our results are consistent with a role for the PRAME ubiquitin ligase complex in NFY-mediated transcriptional regulation.

Originele taal-2	Engels
Pagina's (van-tot)	3786-3798
Aantal pagina's	13
Tijdschrift	EMBO Journal
Volume	30
Nummer van het tijdschrift	18
DOI's	https://doi.org/10.1038/emboj.2011.262
Status	Gepubliceerd - sep. 2011
Extern gepubliceerd	Ja

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10.1038/emboj.2011.262

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Costessi, A., Mahrour, N., Tijchon, E., Stunnenberg, R., Stoel, M. A., Jansen, P. W., Sela, D., Martin-Brown, S., Washburn, M. P., Florens, L., Conaway, J. W., Conaway, R. C., & Stunnenberg, H. G. (2011). The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters. EMBO Journal, 30(18), 3786-3798. https://doi.org/10.1038/emboj.2011.262

@article{72c0819fa7484840bc26d055d43931cd,

title = "The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters",

abstract = "The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumourigenesis remain largely elusive. We applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PRAME can be recruited to DNA in vitro, and genome-wide chromatin immunoprecipitation experiments revealed that PRAME is specifically enriched at transcriptionally active promoters that are also bound by NFY and at enhancers. Our results are consistent with a role for the PRAME ubiquitin ligase complex in NFY-mediated transcriptional regulation.",

keywords = "Cullin2, NFY, PRAME, transcription, ubiquitination",

author = "Adalberto Costessi and Nawel Mahrour and Esther Tijchon and Rieka Stunnenberg and Stoel, {Marieke A.} and Jansen, {Pascal W.} and Dotan Sela and Skylar Martin-Brown and Washburn, {Michael P.} and Laurence Florens and Conaway, {Joan W.} and Conaway, {Ronald C.} and Stunnenberg, {Hendrik G.}",

year = "2011",

month = sep,

doi = "10.1038/emboj.2011.262",

language = "English",

volume = "30",

pages = "3786--3798",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "18",

}

Costessi, A, Mahrour, N, Tijchon, E, Stunnenberg, R, Stoel, MA, Jansen, PW, Sela, D, Martin-Brown, S, Washburn, MP, Florens, L, Conaway, JW, Conaway, RC & Stunnenberg, HG 2011, 'The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters', EMBO Journal, vol. 30, nr. 18, blz. 3786-3798. https://doi.org/10.1038/emboj.2011.262

TY - JOUR

T1 - The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters

AU - Costessi, Adalberto

AU - Mahrour, Nawel

AU - Tijchon, Esther

AU - Stunnenberg, Rieka

AU - Stoel, Marieke A.

AU - Jansen, Pascal W.

AU - Sela, Dotan

AU - Martin-Brown, Skylar

AU - Washburn, Michael P.

AU - Florens, Laurence

AU - Conaway, Joan W.

AU - Conaway, Ronald C.

AU - Stunnenberg, Hendrik G.

PY - 2011/9

Y1 - 2011/9

N2 - The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumourigenesis remain largely elusive. We applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PRAME can be recruited to DNA in vitro, and genome-wide chromatin immunoprecipitation experiments revealed that PRAME is specifically enriched at transcriptionally active promoters that are also bound by NFY and at enhancers. Our results are consistent with a role for the PRAME ubiquitin ligase complex in NFY-mediated transcriptional regulation.

AB - The human tumour antigen PRAME (preferentially expressed antigen of melanoma) is frequently overexpressed in tumours. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumourigenesis remain largely elusive. We applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PRAME can be recruited to DNA in vitro, and genome-wide chromatin immunoprecipitation experiments revealed that PRAME is specifically enriched at transcriptionally active promoters that are also bound by NFY and at enhancers. Our results are consistent with a role for the PRAME ubiquitin ligase complex in NFY-mediated transcriptional regulation.

KW - Cullin2

KW - NFY

KW - PRAME

KW - transcription

KW - ubiquitination

UR - http://www.scopus.com/inward/record.url?scp=80052770792&partnerID=8YFLogxK

U2 - 10.1038/emboj.2011.262

DO - 10.1038/emboj.2011.262

M3 - Article

C2 - 21822215

AN - SCOPUS:80052770792

SN - 0261-4189

VL - 30

SP - 3786

EP - 3798

JO - EMBO Journal

JF - EMBO Journal

IS - 18

ER -

The tumour antigen PRAME is a subunit of a Cul2 ubiquitin ligase and associates with active NFY promoters

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