Third harmonic generation imaging for fast, label-free pathology of human brain tumors

N. V. Kuzmin, P. Wesseling, P. C.de Witt Hamer, D. P. Noske, G. D. Galgano, H. D. Mansvelder, J. C. Baayen, M. L. Groot

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

65 Citaten (Scopus)

Samenvatting

In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an urgent need for tools that provide the neurosurgeon with pathological information during the operation. We show that third harmonic generation (THG) microscopy provides label-free, real-time images of histopathological quality; increased cellularity, nuclear pleomorphism, and rarefaction of neuropil in fresh, unstained human brain tissue could be clearly recognized. We further demonstrate THG images taken with a GRIN objective, as a step toward in situ THG microendoscopy of tumor boundaries. THG imaging is thus a promising tool for optical biopsies.

Originele taal-2Engels
Pagina's (van-tot)1889-1904
Aantal pagina's16
TijdschriftBiomedical Optics Express
Volume7
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 1 mei 2016
Extern gepubliceerdJa

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