Transcription factor achaete-scute homologue 2 initiates follicular T-helper-cell development

Xindong Liu, Xin Chen, Bo Zhong, Aibo Wang, Xiaohu Wang, Fuliang Chu, Roza I. Nurieva, Xiaowei Yan, Ping Chen, Laurens G. Van Der Flier, Hiroko Nakatsukasa, Sattva S. Neelapu, Wanjun Chen, Hans Clevers, Qiang Tian, Hai Qi, Lai Wei, Chen Dong

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

276 Citaten (Scopus)

Samenvatting

In immune responses, activated T cells migrate to B-cell follicles and develop into follicular T-helper (TFH) cells, a recently identified subset of CD4+ T cells specialized in providing help to B lymphocytes in the induction of germinal centres. Although Bcl6 has been shown to be essential in TFH-cell function, it may not regulate the initial migration of Tcells or the induction of the TFH program, as exemplified by C-X-C chemokine receptor type 5 (CXCR5) upregulation4. Here we show that expression of achaete-scute homologue 2 (Ascl2)-a basic helix-loop-helix (bHLH) transcription factor-is selectively upregulated in T FH cells. Ectopic expression of Ascl2 upregulates CXCR5 but not Bcl6, and downregulates C-C chemokine receptor 7 (CCR7) expression in T cells in vitro, as well as accelerating T-cell migration to the follicles and TFH-cell development in vivo in mice. Genome-wide analysis indicates that Ascl2 directly regulates T FH-related genes whereas it inhibits expression of T-helper cell 1 (TH1) and TH17 signature genes. Acute deletion of Ascl2, as well as blockade of its function with the Id3 protein in CD4+ T cells, results in impaired T FH-cell development and germinal centre response. Conversely, mutation of Id3, known to cause antibody-mediated autoimmunity, greatly enhances TFH-cell generation. Thus, Ascl2 directly initiates T FH-cell development.

Originele taal-2Engels
Pagina's (van-tot)513-518
Aantal pagina's6
TijdschriftNature
Volume507
Nummer van het tijdschrift7493
DOI's
StatusGepubliceerd - 2014
Extern gepubliceerdJa

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