Transcriptome analysis using RNA-Seq

Wieteke A.M. Hoeijmakers, Richárd Bártfai, Hendrik G. Stunnenberg

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukpeer review

33 Citaten (Scopus)


Transcriptome analysis by next-generation sequencing (RNA-seq) allows investigation of a transcriptome at unsurpassed resolution. One major benefit is that RNA-seq is independent of a priori knowledge on the sequence under investigation, thereby also allowing analysis of poorly characterized Plasmodium species. Here we provide a detailed protocol for RNA isolation and fragmentation, ribosomal RNA depletion, and cDNA synthesis that enables the preparation of a sequencing library from 1 to 2 m g of total RNA. Although we focus our discussion on the quantitative measurement of gene expression, this protocol is suited for many applications of RNA-seq and allows analysis of most RNA species.

Originele taal-2Engels
SubtitelMethods and Protocols
UitgeverijHumana Press Inc.
Aantal pagina's19
ISBN van geprinte versie9781627030250
StatusGepubliceerd - 2013
Extern gepubliceerdJa

Publicatie series

NaamMethods in Molecular Biology
ISSN van geprinte versie1064-3745


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