Samenvatting
The human DNA-excision repair gene ERCC-1 is cloned by its ability to correct the excision-repair defect of the ultraviolet light- and mitomycin-C-sensitive CHO mutant cell line 43-3B. This mutant is assigned to complementation group 2 of the excision-repair-deficient CHO mutants. In order to establish whether the correlation by ERCC-1 is confined to CHO mutants of one complementation group, the cloned repair gene, present on cosmid 34-43, was transfected to representative cell lines of the 6 complementation groups that have been identified to date. Following transfection, mycophenolic acid was used to select for transferants expressing the dominant marker gene Ecogpt, also present on cosmid 34-43. Cotransfer of the ERCC-1 gene was shown by Southern blot analysis of DNA from pooled (500-2000 independent colonies) transformants of each mutant. UV survival and UV-induced UDS showed that only mutants belonging to complementation group 2 and no mutants of other groups were corrected by the ERCC-1 gene. This demonstrates that ERCC-1 does not provide an aspecific bypass of excision-repair defects in CHO mutants and supports the assumption that the complementation analysis is based on mutations in different repair genes.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 123-130 |
Aantal pagina's | 8 |
Tijdschrift | Mutation Research DNA Repair Reports |
Volume | 193 |
Nummer van het tijdschrift | 2 |
DOI's | |
Status | Gepubliceerd - mrt. 1988 |
Extern gepubliceerd | Ja |