Transforming growth factor beta secretion from primary breast cancer fibroblasts

C. E.P. van Roozendaal; J. G.M. Klijn; B. van Ooijen; C. Claassen; A. M.M. Eggermont; S. C. Henzen-Logmans; J. A. Foekens

doi:10.1016/0303-7207(95)03539-J

Transforming growth factor beta secretion from primary breast cancer fibroblasts

C. E.P. van Roozendaal, J. G.M. Klijn, B. van Ooijen, C. Claassen, A. M.M. Eggermont, S. C. Henzen-Logmans, J. A. Foekens

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Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β₁ has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β₁ were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β₁ secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.

Originele taal-2	Engels
Pagina's (van-tot)	1-6
Aantal pagina's	6
Tijdschrift	Molecular and Cellular Endocrinology
Volume	111
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1016/0303-7207(95)03539-J
Status	Gepubliceerd - 28 apr. 1995
Extern gepubliceerd	Ja

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title = "Transforming growth factor beta secretion from primary breast cancer fibroblasts",

abstract = "Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β1 has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β1 were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β1 secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.",

keywords = "Fibroblast, Human breast cells, Tamoxifen, Transforming growth factor-beta",

author = "{van Roozendaal}, {C. E.P.} and Klijn, {J. G.M.} and {van Ooijen}, B. and C. Claassen and Eggermont, {A. M.M.} and Henzen-Logmans, {S. C.} and Foekens, {J. A.}",

note = "Funding Information: We gratefully acknowledge Maxime P. Look and Wim L.J. van Putten for statistical analysis. This study was supported by the Dutch Cancer Society (grant DDHK 91-15).",

year = "1995",

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doi = "10.1016/0303-7207(95)03539-J",

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AU - van Roozendaal, C. E.P.

AU - Klijn, J. G.M.

AU - van Ooijen, B.

AU - Claassen, C.

AU - Eggermont, A. M.M.

AU - Henzen-Logmans, S. C.

AU - Foekens, J. A.

N1 - Funding Information: We gratefully acknowledge Maxime P. Look and Wim L.J. van Putten for statistical analysis. This study was supported by the Dutch Cancer Society (grant DDHK 91-15).

PY - 1995/4/28

Y1 - 1995/4/28

N2 - Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β1 has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β1 were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β1 secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.

AB - Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β1 has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β1 were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β1 secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.

KW - Fibroblast

KW - Human breast cells

KW - Tamoxifen

KW - Transforming growth factor-beta

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Transforming growth factor beta secretion from primary breast cancer fibroblasts

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