Translational regulation shapes the molecular landscape of complex disease phenotypes

Sebastian Schafer, Eleonora Adami, Matthias Heinig, Katharina E Costa Rodrigues, Franziska Kreuchwig, Jan Silhavy, Sebastiaan van Heesch, Deimante Simaite, Nikolaus Rajewsky, Edwin Cuppen, Michal Pravenec, Martin Vingron, Stuart A Cook, Norbert Hubner

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

The extent of translational control of gene expression in mammalian tissues remains largely unknown. Here we perform genome-wide RNA sequencing and ribosome profiling in heart and liver tissues to investigate strain-specific translational regulation in the spontaneously hypertensive rat (SHR/Ola). For the most part, transcriptional variation is equally apparent at the translational level and there is limited evidence of translational buffering. Remarkably, we observe hundreds of strain-specific differences in translation, almost doubling the number of differentially expressed genes. The integration of genetic, transcriptional and translational data sets reveals distinct signatures in 3'UTR variation, RNA-binding protein motifs and miRNA expression associated with translational regulation of gene expression. We show that a large number of genes associated with heart and liver traits in human genome-wide association studies are primarily translationally regulated. Capturing interindividual differences in the translated genome will lead to new insights into the genes and regulatory pathways underlying disease phenotypes.

Originele taal-2Engels
Pagina's (van-tot)7200
TijdschriftNature communications
Volume6
DOI's
StatusGepubliceerd - 26 mei 2015
Extern gepubliceerdJa

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