Unlike dietary restriction, rapamycin fails to extend lifespan and reduce transcription stress in progeroid DNA repair-deficient mice

María B Birkisdóttir, Dick Jaarsma, Renata M C Brandt, Sander Barnhoorn, Nicole van Vliet, Sandra Imholz, Conny T van Oostrom, Bhawani Nagarajah, Eliana Portilla Fernández, Anton J M Roks, Ype Elgersma, Harry van Steeg, José A Ferreira, Jeroen L A Pennings, Jan H J Hoeijmakers, Wilbert P Vermeij, Martijn E T Dollé

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

Dietary restriction (DR) and rapamycin extend healthspan and life span across multiple species. We have recently shown that DR in progeroid DNA repair-deficient mice dramatically extended healthspan and trippled life span. Here, we show that rapamycin, while significantly lowering mTOR signaling, failed to improve life span nor healthspan of DNA repair-deficient Ercc1∆/- mice, contrary to DR tested in parallel. Rapamycin interventions focusing on dosage, gender, and timing all were unable to alter life span. Even genetically modifying mTOR signaling failed to increase life span of DNA repair-deficient mice. The absence of effects by rapamycin on P53 in brain and transcription stress in liver is in sharp contrast with results obtained by DR, and appoints reducing DNA damage and transcription stress as an important mode of action of DR, lacking by rapamycin. Together, this indicates that mTOR inhibition does not mediate the beneficial effects of DR in progeroid mice, revealing that DR and rapamycin strongly differ in their modes of action.

Originele taal-2Engels
Pagina's (van-tot)e13302
TijdschriftAging cell
Volume20
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - feb. 2021

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