Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains

P. Borst, F. Fase-Fowler, J. H.J. Hoeijmakers, A. C.C. Frasch

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55 Citaten (Scopus)

Samenvatting

We have compared a total of 30 recognition sites for eight restriction endonucleases on the 20-kilobase-pair maxi-circle of kinetoplast DNAs from five different Trypanosoma brucei strains. In addition to three polymorphic sites we have found a 5 kilobase-pair region that is not cleaved by any of the eight enzymes and that varies in size over 1 kilobase pair in the strains analysed. Mini-circles from these five strains, digested with endonuclease TaqI or MboII, yield very complex fragment patterns, showing that extensive mini-circle sequence heterogeneity is a common characteristic of these T. brucei strains. The size distribution of mini-circle fragments in these digests was identical for different clones of the 427 strain, but very different for mini-circles from different strains. These results show that maxi-circle sequence is conserved, whereas mini-circle sequence is not. Restriction digests of maxi-circles could be useful in determining how closely two Trypanosoma strains are related, whereas mini-circle digests can serve as sensitive tags for individual strains.

Originele taal-2Engels
Pagina's (van-tot)197-210
Aantal pagina's14
TijdschriftBBA Section Nucleic Acids And Protein Synthesis
Volume610
Nummer van het tijdschrift2
DOI's
StatusGepubliceerd - 11 dec. 1980
Extern gepubliceerdJa

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