@article{ce92cbfc67f7447a9963edfef5a19777,
title = "XDifferentiated Troy+ chief cells act as reserve stem cells to generate all lineages of the stomach epithelium",
abstract = "Proliferation of the self-renewing epithelium of the gastric corpus occurs almost exclusively in the isthmus of the glands, from where cells migrate bidirectionally toward pit and base. The isthmus is therefore generally viewed as the stem cell zone. We find that the stem cell marker Troy is expressed at the gland base by a small subpopulation of fully differentiated chief cells. By lineage tracing with a Troy-eGFP-ires-CreERT2 allele, single marked chief cells are shown to generate entirely labeled gastric units over periods of months. This phenomenon accelerates upon tissue damage. Troy+ chief cells can be cultured to generate long-lived gastric organoids. Troy marks a specific subset of chief cells that display plasticity in that they are capable of replenishing entire gastric units, essentially serving as quiescent {"}reserve{"} stem cells. These observations challenge the notion that stem cell hierarchies represent a {"}one-way street.{"}",
author = "Stange, {Daniel E.} and Koo, {Bon Kyoung} and Meritxell Huch and Greg Sibbel and Onur Basak and Anna Lyubimova and Pekka Kujala and Sina Bartfeld and Jan Koster and Geahlen, {Jessica H.} and Peters, {Peter J.} and {Van Es}, {Johan H.} and {Van De Wetering}, Marc and Mills, {Jason C.} and Hans Clevers",
note = "Funding Information: This work was supported by grants from the Centre for Biomedical Genetics (CBG) to D.E.S.; the European Research Council (EU/232814-StemCellMark), the National Research Foundation of Korea (NRF2011-357-C00093), and the Wellcome Trust (097922/C/11/Z) to B.-K.K.; an EU Marie Curie Fellowship (EU/236954-ICSC-Lgr5) to M.H.; the Cancer Genomics Center (CGCII) to O.B.; an EU Marie Curie Fellowship (EU/300686-InfO) to S.B.; a grant from TI Pharma (T3-106) to J.H.v.E.; the European Research Council (EU/Health-F4-2007-200720) to M.v.d.W.; and DK094989, 2P30 DK052574 to J.C.M. We would like to thank R. Siegmund for graphical abstract design, Mark J. Miller and the Washington University School of Medicine In vivo Imaging Core (IVIC) for help with two-photon imaging, the Washington University Digestive Disease Core Center (DDRCC) Advanced Imaging and Tissue Analysis Core and Shradha Khurana for help in figure preparation, James Goldenring for constructive discussion, and Frederic J. de Sauvage for Lgr5-DTR:eGFP mice. ",
year = "2013",
month = oct,
day = "10",
doi = "10.1016/j.cell.2013.09.008",
language = "English",
volume = "155",
pages = "357",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "2",
}