Samenvatting
Basic mechanisms of transcription initiation are conserved from yeast to man. However in contrast to genes transcribed by RNA polymerases II and III, ribosomal gene transcription by RNA polymerase I (Pol I) is species-specific. Promoter selectivity is mediated by SL1/TIF-IB, a multiprotein complex containing the TATA-binding protein (TBP) and TBP-associated factors (TAFs) which bind to DNA and nucleate the assembly of initiation complexes. Using a human cell line that expresses epitope-tagged yeast TBP, we have isolated a chimeric complex consisting of yeast TBP and human TAFs which faithfully promotes human rDNA transcription in vitro. This result argues that specific interactions between TBP and Pol I-specific TAFs have been evolutionarily conserved between distant species. In addition, this finding also underscores the importance of TAFs in determining promoter selectivity of Pol I.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 840-845 |
Aantal pagina's | 6 |
Tijdschrift | Journal of Molecular Biology |
Volume | 243 |
Nummer van het tijdschrift | 5 |
DOI's | |
Status | Gepubliceerd - 10 nov. 1994 |
Extern gepubliceerd | Ja |