Yeast TBP can replace its human homologue in the RNA polymerase I-specific multisubunit factor SL1

Udo Rudloff, Hendrik G. Stunnenberg, Marie Keaveney, Ingrid Grummt

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6 Citaten (Scopus)

Samenvatting

Basic mechanisms of transcription initiation are conserved from yeast to man. However in contrast to genes transcribed by RNA polymerases II and III, ribosomal gene transcription by RNA polymerase I (Pol I) is species-specific. Promoter selectivity is mediated by SL1/TIF-IB, a multiprotein complex containing the TATA-binding protein (TBP) and TBP-associated factors (TAFs) which bind to DNA and nucleate the assembly of initiation complexes. Using a human cell line that expresses epitope-tagged yeast TBP, we have isolated a chimeric complex consisting of yeast TBP and human TAFs which faithfully promotes human rDNA transcription in vitro. This result argues that specific interactions between TBP and Pol I-specific TAFs have been evolutionarily conserved between distant species. In addition, this finding also underscores the importance of TAFs in determining promoter selectivity of Pol I.

Originele taal-2Engels
Pagina's (van-tot)840-845
Aantal pagina's6
TijdschriftJournal of Molecular Biology
Volume243
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 10 nov. 1994
Extern gepubliceerdJa

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